Extracellular Matrix Assembly in Diatoms (Bacillariophyceae) (II. 2,6-Dichlorobenzonitrile Inhibition of Motility and Stalk Production in the Marine Diatom Achnanthes longipes)

摘要: The cellulose synthesis inhibitor 2,6-dichlorobenzonitrile (DCB) and the DCB analogs 2-chloro-6-fluorobenzonitrile, 3-amino-2,6-dichlorobenzonitrile, 5-dimethylamino-naphthalene-1-sulfonyl-(3-cyano-2, 4-dichloro)aniline (DCBF) inhibited extracellular adhesive production in marine diatom Achnanthes longipes, resulting a loss of motility lack permanent adhesion. effect was fully reversible upon removal inhibitor, cell growth not affected at concentrations inhibitors adequate to effectively interrupt adhesion sequence. Video microscopy revealed that sequence mediated by export assembly polymers, consisted initial attachment followed eventual structures form stalks elevated above substratum. A. longipes polymers are primarily composed noncellulosic polysaccharides (B.A. Wustman, M.R. Gretz, K.D. Hoagland [1997] Plant Physiol 113: 1059–1069). These results, together with discovery inhibition matrix red algal unicells (S.M. Arad, O. Dubinsky, B. Simon [1994] Phycologia 33: 158–162), indicate inhibits polysaccharides. A fluorescent probe, DCBF, synthesized shown inhibit polymer same manner as DCB. DCBF specifically labeled an 18-kD polypeptide isolated from membrane fraction. Inhibition its provides evidence direct relationship between polysaccharide