An evaluation of the role of leukocytes in the pathogenesis of experimentally induced corneal vascularization. III. Studies related to the vasoproliferative capability of polymorphonuclear leukocytes and lymphocytes.

摘要: Studies in the past have suggested that leukocytes are a prerequisite to corneal vascularization. To test this hypothesis further, experiments were conducted determine whether intracorneal instillation of polymorphonuclear leukocytes, lymphocytes, or components would induce vascular ingrowth. These cells cellular fractions injected intracorneally into Fisher albino rats whose circulating had been depleted by total body x-irradiation. Polymorphonuclear isolated from glycogen-induced peritoneal exudates caused invasion, but lymphocytes obtained thymus, spleen, and lymph nodes failed do so. learn an extractable factor could be these suspended isotonic saline, ultrasonified then centrifuged at 101,952g for 1 hour. Aliquots resulting sediment supernatant with radiation-induced leukopenia. The nonsedimentable vascularization, did not provoke phenomenon. studies only provide further support initiate possibly release one more heat labile chemical mediators, directly implicate leukocyte process.