作者: Alexandru Dumitrache , Gideon M. Wolfaardt , Lee R. Lynd
DOI: 10.1128/9781555816827.CH44
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摘要: In this chapter, biofilm structure and cell behavior in fully hydrated, undisturbed biofilms of Clostridium thermocellum are considered as a means to study formation by anaerobic thermophiles. Recent evidence demonstrates the strong correlation between first-order hydrolysis rates cellulose with concentration sessile bacteria rather than total or planktonic biomass after inoculation enriched leachate rumen fluid. A aimed correlate imaging cellulolytic process performance data real time, results suggested that culture formed thicker more stable inoculum from digester leachate, which were consistent higher solubilization reactors. Cellulolytic organisms occupy special niche where (the primarily) insoluble serves both carbon source solid support, theoretically providing optimal conditions for accumulation preferential access primary substrate. C. is one potential candidates strain development through recombinant classical strategies application consolidated bioprocessing at large-scale conversion renewable into biofuels other products. The numerous examples literature, observed association cells substrate, make organism suitable model delineate cell-cellulosic substratum associations optimize tools phenomenon cultures consortia conversion.