Isolation and nucleotide sequence of the Saccharomyces cerevisiae gene for the succinate dehydrogenase flavoprotein subunit.

摘要: Succinate dehydrogenase (EC 1.3.99.1) of the mitochondrial inner membrane is a four-subunit membrane-bound enzyme that catalyzes oxidation succinate to fumarate and transfer electrons into electron transport chain oxygen. The catalytic domain composed flavoprotein subunit which contains covalently attached FAD cofactor an iron-sulfur with three nonidentical clusters. We have isolated complete genomic clone for from Saccharomyces cerevisiae determined its nucleotide sequence. sequence predicts protein 70,185 Da (640 amino acids) shows more similarity Escherichia coli than it does only other homologue, human subunit. yeast precursor was synthesized in cell-free translation system shown possess targeting directs import isolated, energized mitochondria where processed by matrix-localized protease. genes subunits reside on different chromosomes hence form transcriptional units.