Dual physiological effects of antifungal sterol biosynthetic inhibitors on enzyme targets and on transcriptional regulation

摘要: For many antifungal agents, enzymes leading to ergosterol biosynthesis are primary targets. Inhibition of in treated cells results the formation aberrant sterols, lacking one or more structural features ergosterol. Furthermore, total sterol levels often higher than amounts non-treated cells. The ERG3 gene, encoding C-5 desaturase, was used as a model for regulation by some agents genes biosynthesis. Treatment yeast with three biosynthetic inhibitors different targets pathway led an increase mRNA levels. drug treatment correlated decrease content within No correlation evident between and levels, at least inhibitor, fenpropimorph, slight sterol, while decreased. fenpropimorph ketoconazole resulted percentage lovastatin caused percentage. These indicate that second indirect effect is on transcriptional regulation. physiology cell affected not only but also enhanced accumulation defective sterols.