Neutral metalloendopeptidase in human lung tissue and cultured cells.
作者： Alice R. Johnson , Ervin G. Erdös , Werner W. Schulz , Juliet Ashton
DOI: 10.1164/ARRD.1985.132.3.564
关键词:
摘要: The distribution of a neutral metalloendopeptidase (NEP), or “enkephalinase,” in human lung tissue and cultured cells was compared with that angiotensin I converting enzyme (ACE). specific activities NEP ACE were measured homogenates fetal isolated airways pulmonary vessels. activity highest airway tissue, Human endothelial from either umbilical veins arteries had high (80 to 90 nmol/h/106 cells) but only trace (0.5 0.6 cells). Fibroblasts lungs low richer than cells. foreskins caesarean section skin the richest source (60 80 Immunohistochemical studies confirmed biochemical assays. As expected, localized on luminal surface blood vessels, similar factor VIII an...
sci-hub.se 参考文章(28)
M. A. Kerr, A. J. Kenny, The purification and specificity of a neutral endopeptidase from rabbit kidney brush border. Biochemical Journal. ,vol. 137, pp. 477- 488 ,(1974) , 10.1042/BJ1370477
C B Couch, W J Strittmatter, Specific blockers of myoblast fusion inhibit a soluble and not the membrane-associated metalloendoprotease in myoblasts. Journal of Biological Chemistry. ,vol. 259, pp. 5396- 5399 ,(1984) , 10.1016/S0021-9258(18)91021-5
Alice R. Johnson, Human Pulmonary Endothelial Cells in Culture Journal of Clinical Investigation. ,vol. 65, pp. 841- 850 ,(1980) , 10.1172/JCI109736
Cesar Labarca, Kenneth Paigen, A simple, rapid, and sensitive DNA assay procedure. Analytical Biochemistry. ,vol. 102, pp. 344- 352 ,(1980) , 10.1016/0003-2697(80)90165-7
John A. Weare, John T. Gafford, H.S. Lu, Ervin G. Erdös, Purification of human kidney angiotensin I converting enzyme using reverse-immunoadsorption chromatography. Analytical Biochemistry. ,vol. 123, pp. 310- 319 ,(1982) , 10.1016/0003-2697(82)90451-1
J.P. Swerts, R. Perdrisot, B. Malfroy, J.C. Schwartz, Is ‘enkephalinase” identical with ‘angiotensin-converting enzyme’? European Journal of Pharmacology. ,vol. 53, pp. 209- 210 ,(1979) , 10.1016/0014-2999(79)90167-5
P. Caldwell, B. Seegal, K. Hsu, M Das, R. Soffer, Angiotensin-converting enzyme: vascular endothelial localization Science. ,vol. 191, pp. 1050- 1051 ,(1976) , 10.1126/SCIENCE.175444
Ervin G. Erdös, Alice R. Johnson, Nit T. Boyden, Hydrolysis of enkephalin by cultured human endothelial cells and by purified peptidyl dipeptidase Biochemical Pharmacology. ,vol. 27, pp. 843- 848 ,(1977) , 10.1016/0006-2952(78)90542-7
Koon Sea Hui, You Jing Wang, Abel Lajtha, Purification and characterization of an enkephalin aminopeptidase from rat brain membranes. Biochemistry. ,vol. 22, pp. 1062- 1067 ,(1983) , 10.1021/BI00274A010
John T. Gafford, Randal A. Skidgel, Ervin G. Erdos, Louis B. Hersh, Human kidney "enkephalinase", a neutral metalloendopeptidase that cleaves active peptides Biochemistry. ,vol. 22, pp. 3265- 3271 ,(1983) , 10.1021/BI00282A035