5-Azacytidine treatment induces demethylation of DAPK1 and MGMT genes and inhibits growth in canine mammary gland tumor cells.

摘要: Background Canine mammary gland tumors (CMGTs) are the most common, spontaneous types of neoplasias in female dogs. Aberrant DAPK1 and MGMT methylation associated with tumor formation development various cancers. 5-Azacytidine is a known specific demethylation drug that covalently binds to DNA methyltransferase. However, unknown respect CMGTs. Therefore, we sought demonstrate effects 5-azacytidine on proliferation CMGTs cell, elucidate potential molecular mechanisms action these cancerous cells. Materials methods The CHMm CHMp cell were evaluated by MTT assay. gene patterns cells blood/tissue samples analyzed MSP Effect gene, mRNA expression assay qRT-PCR assay, respectively. Results may suppress Furthermore, genes hypermethylated CHMm/CHMp clinical malignant samples, but not normal dogs' blood tissue. re-inducible treated 5 μM 5-azacytidine. Meanwhile, increased mRNA. Conclusion These results suggest can serve as sensitive diagnostic biomarkers therapeutic targets for also could be candidate