Cellular distribution of ferric iron, ferritin, transferrin and divalent metal transporter 1 (DMT1) in substantia nigra and basal ganglia of normal and beta2-microglobulin deficient mouse brain.

摘要: We examined whether high levels of circulatory iron may cause accumulation in the brain. In particular, we focussed on substantia nigra and basal ganglia as several papers have indicated that accumulate here death dopaminergic neurons. Normal mice a mouse model hereditary haemochromatosis, beta2-microglobulin (beta2m) knock out [beta2m (-/-)] mouse, which has circulating due to increased absorption, were examined. The concentration livers were: 170+/-15 microg/g (mean +/- SD) controls 1010+/-50 beta2m (-/-) (p<0.001), whereas brain respective values 47 +/-1 53+/-2 (p<0.02). Hence, difference between cerebral normal was small. Histological examination brains revealed an unequivocal distribution ferric iron, ferritin, transferrin divalent metal transporter 1 (DMT1), indistinguishable when compared. ganglia, iron-binding proteins present identical cell types, mainly comprised oligodendrocytes microglia. Neurons lightly labelled with DMT1. virtual lack increase clearly shows blood-brain barrier (BBB) is capable restricting transport excess plasma into