Multistage-multiorifice flow fractionation (MS-MOFF): continuous size-based separation of microspheres using multiple series of contraction/expansion microchannels

摘要: Previously we introduced a novel hydrodynamic method using multi-orifice microchannel for size-based particle separation, which is called flow fractionation (MOFF). The MOFF has several advantages such as continuous, non-intrusive, and minimal power consumption. However, it limitation that the recovery yield relatively low. Although may be increased by adjusting parameters Reynolds number central collecting region, poor purity inevitably followed. We newly designed fabricated microfluidic channel multi-stage (MS-MOFF), made combining three segments, consists of 3 inlets, filters, segments 5 outlets. structure dimensions MS-MOFF were determined principles to have constant numbers at each segment. Polystyrene microspheres two different sizes (7 μm 15 μm) tested. With this device, an attempt improve minimize loss re-separating non-selected particles first separation. final successfully from 73.2% 88.7% while slightly decreased 91.4% 89.1% (for μm). These values never achievable with single-stage (SS-MOFF) having only one segment in our previous work. will useful clinical applications, separation circulating tumor cells (CTC) or rare human blood samples.