Molecular cloning and expression of two alpha-amylase genes from Streptococcus bovis 148 in Escherichia coli
作者： E Satoh , Y Niimura , T Uchimura , M Kozaki , K Komagata
DOI: 10.1128/AEM.59.11.3669-3673.1993
关键词:
摘要: The alpha-amylase genes of Streptococcus bovis 148 were cloned in Escherichia coli MC1061, using pBR322. recombinant plasmids classified into two groups on the basis their restriction maps. Southern blot analysis did not show homology between types genes, and existed chromosomal DNA S. 148. enzymatic properties N-terminal amino acid sequences purified enzymes produced by E. strains quite different from each other. Particularly, one (Amy I) was adsorbed raw corn starch hydrolyzed starch, another II) hydrolyze starch. Amy I considered to be same as extracellular absorbability, ability characteristics, sequence, mode action soluble II showed a unique pattern oligosaccharide production compared with suggested an intracellular
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