siRNA delivery with chitosan nanoparticles: Molecular properties favoring efficient gene silencing.
作者： Jostein Malmo , Hanne Sørgård , Kjell M. Vårum , Sabina P. Strand
DOI: 10.1016/J.JCONREL.2011.11.012
关键词:
摘要: Abstract Chitosan has gained increasing interest for siRNA delivery. Although chitosan covers a family of structurally different polysaccharides, most delivery studies have been performed with conventional partially N -acetylated chitosans. Herein, the purpose was to identify fundamental molecular properties favoring and efficient gene silencing in mammalian cells. Nanoparticles were prepared from well-defined chitosans various chemical compositions, degrees polymerization (DP n ) chain architectures. Structure-activity relationships determined by cellular uptake knockdown efficiency at mRNA protein levels. Additionally, nanoparticle cytotoxicity evaluated on basis metabolic activity membrane integrity. Our results show that achieved using fully de- intermediate lengths 100–300). These mediated low concentrations and, several cell lines, potent long-term both exogenous endogenous target genes, minimal cytotoxicity.
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