Cloning and characterization of the wheat domestication gene, Q

摘要: The Q gene is largely responsible for the widespread cultivation of durum and common wheat because it confers square spike phenotype free-threshing character. It also pleiotropically influences many other domestication related traits such as glume shape, tenacity, rachis fragility, length, plant height, emergence time. objectives this research were to confirm or reject hypothesis that a candidate AP2-like Q, dosage pleiotropic effects attributed begin defining differences between q alleles. identity was verified by analysis knockout mutants found have high degree similarity members AP2 family transcription factors. Southern multiple Triticum taxa containing either indicated locus not composed duplicated Ectopic expression allowed observation both silencing over-expression Q. Rachis tenacity mimicked in transgenic plants exhibiting transcriptional transgene endogenous gene. Variation compactness height directly associated with level due Comparisons structural well variation transcription. One amino acid difference several base changes within promoter identified possible critical q. Very little genetic variability sequenced alleles suggesting arose only once more primitive allele. Cloning Characterization Wheat Domestication Gene,