Proteins of crustacean exoskeleton: IV. Partial amino acid sequences of exoskeletal proteins from the Bermuda land crab, Gecarcinus lateralis, and comparisons to certain insect proteins.

摘要: As in all decapod Crustacea, the exoskeleton of land crab Gecarcinus lateralis consists four layers. Prior electrophoretic analysis proteins extracted from these layers revealed an abundance small Mr with acidic pIs as are found insect cuticle (O'Brien et al. [1991] Biol. Bull., 181:427–441). Further, immunological cross-reactivity between exoskeletal and cuticular has been demonstrated (Kumari Skinner [1993] J. Exp. Zool., 265:195–210). Partial amino acid sequences a number described here. Proteins were electrophoresed on two-dimensional (2D) gels, transferred to polyvinylidene difluoride (PVDF) membranes, stained; individual spots recovered their N-termini sequenced. In addition, 14-kDa protein (pI =5.4) membranous layer (ML14) was eluted 2D gels digested endoproteinase Lys-C; its constituent peptides sequenced. The two epicuticular differed each other. Three identical mobility isolated exocuticle, endocuticle, appeared have N termini, while another electrophoretically set three other but positions first set. Two both had mass 25 kDa different isoelectric points. Their indistinguishable clearly distinct protein. Another sequence less pair endocuticle quite similar one another. The internal peptide fragments ML14 distinct, regions terminus. One some structural vertebrates, whereas others motifs proteins. similarities identified did not account for antibody cross-reactivity. © 1995 Wiley-Liss, Inc.