Design and fabrication of a fully-compliant mechanism for control of cellular injection arrays

摘要: This paper reports on the design, fabrication, and testing of a fully-compliant mechanism designed for precise parallel guidance. The is to guide arrays micro-scale needles straight-line injections thousands culture cells simultaneously. During injection, needle array be lowered into cell dish. It must guided carefully during insertion because any rotation or transverse motion can lead tearing delicate membranes, leading death. injection system consists motion, while resisting motions in other direction. Successive prototypes are shown illustrate process benefits final system. Prototypes were demonstrated using deep reactive ion etching silicon wafers, as well rapid prototyping three-dimensional printer ABS polymer. single, 3-D-printed part that incorporates compliance over distance about 1.5 mm restraining deflections rotations. Testing shows performed result high viability, suggesting appropriately restrains off-axis motions. While here specifically biological research, it may adapted manufacturing, position control, application requiring minimizing