Polypyrrole-based potentiometric biosensor for urea part 1. Incorporation of urease

作者: S.B. Adeloju , S.J. Shaw , G.G. Wallace

DOI: 10.1016/0003-2670(93)85022-C

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摘要: Abstract A method is described for the incorporation of urease into a polypyrrole film by galvanostatic polymerisation on gold-coated plastic films and solid gold electrodes. The presence in was verified x-ray photoelectron spectroscopy, amino acid analysis, scanning microscopy cyclic voltammetry. enzyme activity polypyrrole-urease confirmed its catalytic conversion urea to carbon dioxide ammonia. use both spectrophotometric potentiometric methods detection their potential determination discussed.

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