Endogenous cellulase enzymes in the stick insect (Phasmatodea) gut.
作者： Matan Shelomi , Hirofumi Watanabe , Gaku Arakawa
DOI: 10.1016/J.JINSPHYS.2013.10.007
关键词:
摘要: High cellulase (endo-beta-1,4-glucanase) activity was detected in the anterior midgut of walking stick (Phasmatodea) Eurycantha calcarata. The enzyme isolated and analyzed via mass spectrometry. RT-PCR revealed two endoglucanase genes, EcEG1 EcEG2. Mascot analysis purified confirms it to be product gene EcEG1. Homologous cDNAs were also from a distantly related species, Entoria okinawaensis, suggesting general distribution genes phasmids. Phasmid cellulases showed high homology endogenously-produced glycoside hydrolase family 9 (GH9) endoglucanases insects, especially those termites, cockroaches, crickets. E. calcarata clear antigency against an anti-serum for termite GH9 cellulase, which, together with sequence homology, further suggests endogenous origin enzyme. This discovery possible nutritive value cellulose leaf-feeding phasmids, unlike herbivorous Lepidoptera.
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