Nucleotide sequence of cloned cDNA of human c- myc oncogene

摘要: Like other transforming genes of retro viruses, the v-myc gene avian virus, MC29, has a homologue in genome normal eukaryotic cells. The human cellular homologue, c-myc, located on chromosome 8, region q24→qter (refs 1, 2), is translocated into immunoglobulin heavy-chain locus 14 (ref. 3) Burkitt's lymphoma1,4,5, suggesting that c-myc primary role transformation some haematopoietic In addition, amplified promyelocytic leukaemia cell line, HL60 6, 7) which also contains high levels mRNA8. Recently, Colby et al.9 reported nucleotide sequence DNA isolated from genomic recombinant library derived fetal liver10. This 4,053-base pair (bp) includes two exons and one intron myc gene, authors have suggested existence mRNA 2,291 nucleotides coding capacity for protein molecular weight (Mr) 48,812. We approached problem accurately defining characteristics by determining cDNA prepared clone K562 leukaemic line11. cells are known to contain similar size types8. report here 2,121 demonstrate its 5′ noncoding does not correspond sequence. However, our data confirm intact can encode 48,812-Mr with identical al.9.