Modulated molecular markers of restenosis and thrombosis by in-vitro vascular cells exposed to bioresorbable scaffolds.

摘要: Drug-eluting Bioresorbable Vascular Scaffolds (BVS) have emerged as a potential breakthrough for the treatment of coronary artery stenosis, providing mechanical support and drug delivery followed by complete resorption. Restenosis thrombosis remain primary limitations in clinical use. The study aimed to identify markers restenosis analyzing vascular wall cell transcriptomic profile modulation triggered BVS at different values shear stress. Human Coronary Artery Endothelial Cells (HCAECs) Smooth Muscle (HCASMCs) were cultured under stress (SS) (1 20 dyne/cm2) 6 hours without with application Everolimus 600 nM. Cell RNA-Seq bioinformatics analysis identified modulated genes direct comparison conditions Gene Ontology (GO). results experimental GO highlighted specific SEMA3E (Semaphorin 3E), MEOX2 (Mesenchyme Homeobox 2), BMP4 (Bone Morphogenetic Protein 4), HMOX1 (Heme Oxygenase 1) SELE (Selectin E), roles pathological evolution disease. Transcriptomic dynamic cultures identifies candidate related pro-restenotic pro-thrombotic mechanisms an in-vitro setting BVS, which are not adequately contrasted addition.