Effects of the G-Protein β3Subunit 825T Allele on Adipogenesis and Lipolysis in Cultured Human Preadipocytes and Adipocytes

摘要: The recently discovered C825T polymorphism of the G-protein beta 3 subunit has been reported to be associated with development hypertension and obesity. aim our study was investigate relationship between functional aspects human adipose cells, particularly regard differentiation lipolysis. Adipose tissue samples were collected from 65 women a BMI ranging 19.7 39.7 kg/m 2 undergoing surgical mammary reduction. stromal cells allowed undergo in primary culture using adipogenic media defined composition. No significant difference observed CC carriers T allele under all conditions capacity related genotype. In subgroup patients (n = 20), lipolysis isolated fat determined by measurement glycerol medium upon catecholamine exposure. Glycerol release after 10(-7) mmol/l isoproterenol significantly higher 10 than adipocytes when expressed as percentage basal (increase above baseline: CC: 809 +/- 174 %, carriers: 247 88 p 0.01), while concentrations no different according genotype controlling for either age or BMI. conclusion, this provides first evidence that GNB3 825T is an impairment beta-adrenergic control