Methanogenic archaea and sulfate reducing bacteria co-cultured on acetate: teamwork or coexistence?

摘要: Acetate is a major product of fermentation processes and an important substrate for sulfate reducing bacteria methanogenic archaea. Most studies on acetate catabolism by reducers methanogens have used pure cultures. Less known about conversion mixed cultures the interactions between both groups. We tested interspecies hydrogen transfer coexistence marine using two types microorganisms. First, Desulfovibrio vulgaris subsp. (DSM 1744), hydrogenotrophic reducer, was cocultured together with obligate aceticlastic methanogen Methanosaeta concilii as carbon energy source. Next, Methanococcus maripaludis S2, H2- formate-utilizing methanogen, partner organism to M. in presence acetate. Finally, we performed experiment acetotrophic reducer Desulfobacter latus AcSR2. Our results showed that D. able reduce grow from leaked concilii. In other coculture, sustained revealed qPCR. The growth microbes indicated co-existence rather than competition. Altogether, our indicate H2 leaking could be efficient H2-scavengers. This metabolic trait, coculture studies, brings new insight flexibility residing environments response changing environmental conditions community compositions. Using dedicated physiological were unravel occurrence less obvious sulfate-reducing bacteria.