Production and characterization of antibodies against vaccinia virus DNA polymerase.

摘要: Poxviruses are large DNA viruses that replicate in discrete locations the cytoplasm of infected cells called viral factories. Because host cell replication machinery is located nucleus, poxviruses encode many proteins required for their own replication, including a polymerase. Although if not all enzymes have been identified, actual mechanism poxvirus remains unclear. Two monoclonal antibodies and polyclonal antibody against vaccinia virus polymerase were produced characterized use as tools to investigate replication. suitable Western blotting, was able detect protein lysates using this method. In contrast, while did recognize when used immunofluorescence microscopy, addition, one these also stained factories by cowpox ectromelia, two closely related viruses. Finally, three immunoprecipitate from lysates. These will be useful experiments designed describe more fully role virus.