A putative ATP-activated Na+ channel involved in sperm-induced fertilization
作者： Y Kupitz , D Atlas
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摘要: Extracellular application of adenosine triphosphate (ATP) to defolliculated Xenopus laevis oocytes activated a saturating inward current with maximal amplitude E(max) 2.4 +/- 0.2 microamperes and an apparent Michaelis constant 197.6 micromolar. The was carried predominantly by sodium ions potently inhibited amiloride, guanosine (GTP), its nonhydrolyzable analogs 5'-[beta,gamma-imido]triphosphate (GppNHp) 5'-O-(3-thiotriphosphate). Likewise, in vitro fertilization using mature eggs sperm GTP, GppNHp. Hence, ATP receptor on the egg membrane may be recipient target for originating sperm, suggesting that ATP-induced increase permeability mediates initial signal process.
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Bruce P. Bean, David D. Friel, ATP-Activated Channels in Excitable Cells Ion channels. ,vol. 2, pp. 169- 203 ,(1990) , 10.1007/978-1-4615-7305-0_5
D. Sela, E. Ram, D. Atlas, ATP receptor. A putative receptor-operated channel in PC-12 cells Journal of Biological Chemistry. ,vol. 266, pp. 17990- 17994 ,(1991) , 10.1016/S0021-9258(18)55227-3
RI Hume, MG Honig, Excitatory action of ATP on embryonic chick muscle The Journal of Neuroscience. ,vol. 6, pp. 681- 690 ,(1986) , 10.1523/JNEUROSCI.06-03-00681.1986
R Neuhaus, BF Reber, H Reuter, Regulation of bradykinin- and ATP-activated Ca(2+)-permeable channels in rat pheochromocytoma (PC12) cells The Journal of Neuroscience. ,vol. 11, pp. 3984- 3990 ,(1991) , 10.1523/JNEUROSCI.11-12-03984.1991
David Epel, The initiation of development at fertilization (Review) Mechanisms of Development. ,vol. 29, pp. 1- 12 ,(1990) , 10.1016/0922-3371(90)90019-S
K Nakazawa, K Fujimori, A Takanaka, K Inoue, An ATP-activated conductance in pheochromocytoma cells and its suppression by extracellular calcium. The Journal of Physiology. ,vol. 428, pp. 257- 272 ,(1990) , 10.1113/JPHYSIOL.1990.SP018211
Frank J. Longo, John W. Lynn, David H. McCulloh, Edward L. Chambers, Correlative ultrastructural and electrophysiological studies of sperm-egg interactions of the sea urchin, Lytechinus variegatus Developmental Biology. ,vol. 118, pp. 155- 166 ,(1986) , 10.1016/0012-1606(86)90083-7
Herbert Schuel, Regina Schuel, A rapid sodium-dependent block to polyspermy in sea urchin eggs Developmental Biology. ,vol. 87, pp. 249- 258 ,(1981) , 10.1016/0012-1606(81)90148-2
B.D. Gomperts, J.M. Fernandez, Techniques for membrane permeabilization Trends in Biochemical Sciences. ,vol. 10, pp. 414- 417 ,(1985) , 10.1016/0968-0004(85)90020-9
C D Benham, T B Bolton, N G Byrne, W A Large, Action of externally applied adenosine triphosphate on single smooth muscle cells dispersed from rabbit ear artery. The Journal of Physiology. ,vol. 387, pp. 473- 488 ,(1987) , 10.1113/JPHYSIOL.1987.SP016585