Reduced hTERT protein levels are associated with DNA aneuploidy in the colonic mucosa of patients suffering from longstanding ulcerative colitis

作者: MARIANN FRIIS-OTTESSEN , PAULA M. DE ANGELIS , AASA R. SCHJØLBERG , SOLVEIG N. ANDERSEN , OLE PETTER F. CLAUSEN

DOI: 10.3892/IJMM.2014.1708

关键词:

摘要: Longstanding ulcerative colitis (UC) is a disease of chronic inflammation the colon. It associated with development colorectal cancer through multistep process including increasing degrees dysplasia and DNA-ploidy changes. However, not all UC patients will develop these characteristics even during lifelong disease, may therefore be divided into progressors who or cancer, non-progressors do exhibit such In present study, amount hTERT, catalytic subunit enzyme telomerase, was estimated by using peroxidase immunohistochemistry (IHC) in set progressor non-progressor colectomies. The protein levels colonic mucosa were compared, further comparisons between different categories dysplastic to status within made. Levels hTERT elevated when compared non-UC control samples, but no difference observed non-progressors. Ki67 significant degree expression lesions DNA-aneuploidy decreased as diploid lesions, stratified for classes morphology. Our results indicate an association aneuploidy UC-progressor colons, also possible protective mechanism Ki67, against malignant features UC-colon.

参考文章(41)
Silvia Bacchetti, Hal W. Hirte, Kazy Hay, Steven Gallinger, Corinne Chadeneau, Telomerase activity associated with acquisition of malignancy in human colorectal cancer. Cancer Research. ,vol. 55, pp. 2533- 2536 ,(1995)
A. Roessner, D. Kuester, P. Malfertheiner, R. Schneider-Stock, Oxidative stress in ulcerative colitis-associated carcinogenesis. Pathology Research and Practice. ,vol. 204, pp. 511- 524 ,(2008) , 10.1016/J.PRP.2008.04.011
Alexander H. Moskovitz, David A. Crispin, John D. Potter, Peter S. Rabinovitch, Jacintha N. O'Sullivan, Mary P. Bronner, Teresa A. Brentnall, Jennifer C. Finley, Wen-Tang Shen, Scott Emerson, Mary J. Emond, Katherine A. Gollahon, Chromosomal instability in ulcerative colitis is related to telomere shortening. Nature Genetics. ,vol. 32, pp. 280- 284 ,(2002) , 10.1038/NG989
Elke Kleideiter, Ulrike Friedrich, Alexandra Möhring, Siegfried Walker, Emil Höring, Karlernst Maier, Peter Fritz, Klaus-Peter Thon, Ulrich Klotz, Telomerase activity in chronic inflammatory bowel disease. Digestive Diseases and Sciences. ,vol. 48, pp. 2328- 2332 ,(2003) , 10.1023/B:DDAS.0000007871.86129.4A
GI Meling, OPF Clausen, A Bergan, A Schjølberg, TO Rognum, Flow cytometric DNA ploidy pattern in dysplastic mucosa, and in primary and metastatic carcinomas in patients with longstanding ulcerative colitis. British Journal of Cancer. ,vol. 64, pp. 339- 344 ,(1991) , 10.1038/BJC.1991.302
Jesse J. Salk, Aasthaa Bansal, Lisa A. Lai, David A. Crispin, Cigdem H. Ussakli, Marshall S. Horwitz, Mary P. Bronner, Teresa A. Brentnall, Lawrence A. Loeb, Peter S. Rabinovitch, Rosa Ana Risques, Clonal expansions and short telomeres are associated with neoplasia in early-onset, but not late-onset, ulcerative colitis Inflammatory Bowel Diseases. ,vol. 19, pp. 2593- 2602 ,(2013) , 10.1097/MIB.0B013E3182A87640
Rosa Ana Risques, Lisa A. Lai, Cigdem Himmetoglu, Anoosheh Ebaee, Lin Li, Ziding Feng, Mary P. Bronner, Bassel Al-Lahham, Kris V. Kowdley, Keith D. Lindor, Peter S. Rabinovitch, Teresa A. Brentnall, Ulcerative Colitis–Associated Colorectal Cancer Arises in a Field of Short Telomeres, Senescence, and Inflammation Cancer Research. ,vol. 71, pp. 1669- 1679 ,(2011) , 10.1158/0008-5472.CAN-10-1966
Bernhard Usselmann, Mark Newbold, Alan G Morris, Chuka U Nwokolo, Deficiency of colonic telomerase in ulcerative colitis The American Journal of Gastroenterology. ,vol. 96, pp. 1106- 1112 ,(2001) , 10.1111/J.1572-0241.2001.03752.X