[2] Structural analysis of NADH: Ubiquinone oxidoreductase from bovine heart mitochondria
作者： John E. Walker , J. Mark Skehel , Susan K. Buchanan
DOI: 10.1016/0076-6879(95)60127-9
关键词:
摘要: Publisher Summary This chapter discusses the chromatographic isolation of bovine complex I, as is a modification that permits both I and adenosine triphosphate (ATP) synthase to be recovered purified from same mitochondrial preparation. The hydrophobic subunits were extracted enzyme with organic solvents. Methods have been designed for extraction larger smaller samples respectively. chloroform used in these procedures was prewashed water. With chloroform: methanol, selective achieved only by Hatefi procedure not either prepared or subcomplexes Iα Iβ, probably because detergent samples. components extracts identified protein sequencing. Details splitting detergents Iβ are also given, subunit compositions, activities, electron paramagnetic resonance (EPR) spectra summarized.
sci-hub.se 参考文章(28)
A Dupuis, J M Skehel, J E Walker, NADH:ubiquinone oxidoreductase from bovine mitochondria. cDNA sequence of a 19 kDa cysteine-rich subunit Biochemical Journal. ,vol. 277, pp. 11- 15 ,(1991) , 10.1042/BJ2770011
Y. Hatefi, A.G. Haavik, D.E. Griffiths, Studies on the electron transfer system. XL. Preparation and properties of mitochondrial DPNH-coenzyme Q reductase. Journal of Biological Chemistry. ,vol. 237, pp. 1676- 1680 ,(1962) , 10.1016/S0021-9258(19)83761-4
C. IAN RAGAN, Structure of NADH-Ubiquinone Reductase (Complex I) Current Topics in Bioenergetics. ,vol. 15, pp. 1- 36 ,(1987) , 10.1016/B978-0-12-152515-6.50005-9
John E. Walker, Jesús M. Arizmendi, Alain Dupuis, Ian M. Fearnley, Moshe Finel, Simon M. Medd, Stephanie J. Pilkington, Michael J. Runswick, J. Mark Skehel, Sequences of 20 subunits of NADH : ubiquinone oxidoreductase from bovine heart mitochondria : application of a novel strategy for sequencing proteins using the polymerase chain reaction Journal of Molecular Biology. ,vol. 226, pp. 1051- 1072 ,(1992) , 10.1016/0022-2836(92)91052-Q
I M Fearnley, M Finel, J M Skehel, J E Walker, NADH:ubiquinone oxidoreductase from bovine heart mitochondria. cDNA sequences of the import precursors of the nuclear-encoded 39 kDa and 42 kDa subunits. Biochemical Journal. ,vol. 278, pp. 821- 829 ,(1991) , 10.1042/BJ2780821
C Paech, A Friend, T P Singer, Simplified isolation and molecular composition of NADH dehydrogenase of the respiratory chain. Biochemical Journal. ,vol. 203, pp. 477- 481 ,(1982) , 10.1042/BJ2030477
Walter H. Vanneste, The stoichiometry and absorption spectra of components a and a-3 in cytochrome c oxidase. Biochemistry. ,vol. 5, pp. 838- 848 ,(1966) , 10.1021/BI00867A005
Hermann Schägger, Gebhard von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry. ,vol. 166, pp. 368- 379 ,(1987) , 10.1016/0003-2697(87)90587-2
Michael J. Runswick, Ian M. Fearnley, J.Mark Skehel, John E. Walker, Presence of an acyl carrier protein in NADH:ubiquinone oxidoreductase from bovine heart mitochondria. FEBS Letters. ,vol. 286, pp. 121- 124 ,(1991) , 10.1016/0014-5793(91)80955-3
Moshe Finel, J. Mark Skehel, Simon P. J. Albracht, Ian M. Fearnley, John E. Walker, Resolution of NADH:ubiquinone oxidoreductase from bovine heart mitochondria into two subcomplexes, one of which contains the redox centers of the enzyme. Biochemistry. ,vol. 31, pp. 11425- 11434 ,(1992) , 10.1021/BI00161A022