Cellobiose dehydrogenase modified electrodes: advances by materials science and biochemical engineering

摘要: The flavocytochrome cellobiose dehydrogenase (CDH) is a versatile biorecognition element capable of detecting carbohydrates as well quinones and catecholamines. In addition, it can be used an anode biocatalyst for enzymatic biofuel cells to power miniaturised sensor–transmitter systems. Various electrode materials designs have been tested in the past decade utilize enhance direct electron transfer (DET) from enzyme electrode. Additionally, mediated (MET) approaches via soluble redox mediators polymers pursued. Biosensors cellobiose, lactose glucose determination are based on CDH different fungal producers, which show differences with respect substrate specificity, pH optima, DET efficiency surface binding affinity. detection catecholamines use analyte regeneration signal amplification. This review discusses sensitivity selectivity CDH-based biosensors, focus (1) more efficient chemically modified or nanostructured electrodes, (2) synthesis custom-made higher MET currents (3) engineering enzymes reaction pathways. Combination these strategies will enable design sensitive selective biosensors reduced size analytes continuous on-site point-of-care applications.