Involvement of an enterocyte renin-angiotensin system in the local control of SGLT1-dependent glucose uptake across the rat small intestinal brush border membrane.

摘要: There is increasing evidence that locally produced angiotensin AII (AII) regulates the function of many tissues, but involvement enterocyte-derived in control intestinal transport unknown. This study examined whether there a local renin–angiotensin system (RAS) rat villus enterocytes and assessed effects on SGLT1-dependent glucose across brush border membrane (BBM). Gene protein expression angiotensinogen, ACE, AT1 AT2 receptors were studied jejunal ileal using immunocytochemistry, Western blotting RT-PCR. Mucosal uptake d-[14C]glucose by everted sleeves before after addition (0–100 nm) to mucosal buffer was measured presence or absence receptor antagonist losartan (1 μm). Immunocytochemistry revealed enterocytes; immunoreactivity angiotensinogen proteins especially pronounced at BBM. Expression receptors, not greater ileum than jejunum. Addition inhibited phlorizin-sensitive (SGLT1-dependent) rapid dose-dependent manner reduced SGLT1 Losartan attenuated inhibitory action uptake. did affect l-leucine. The detection RAS components enterocyte BBM, inhibition luminal suggest secretion exerts autocrine transport.