A guide for measurement of circulating metabolic hormones in rodents: Pitfalls during the pre-analytical phase

摘要: Researchers analyse hormones to draw conclusions from changes in hormone concentrations observed under specific physiological conditions and elucidate mechanisms underlying their biological variability. It is, however, frequently overlooked that also circumstances occurring after collection of samples can significantly affect the measured, owing analytical pre-analytical Whereas awareness for such potential confounders is increasing human laboratory medicine, there sometimes limited consensus about control these factors rodent studies. In this guide, we demonstrate how reliability consequent interpretation data immunoassay measurements circulating metabolic We compare knowledge studies recent recommendations established biomarker humans give practical rodents.