Antimicrobial susceptibility of multidrug-resistant Pseudomonas aeruginosa isolated from drinking water and hospitalized patients in Jordan

摘要: Abstract Pseudomonas aeruginosa (P. aeruginosa) is an important environmental, opportunistic and nosocomial pathogen with a significant threat to public health. The objectives of this study were determine the in vitro antimicrobial susceptibility patterns of, antibiotic drug combinations synergistic effects against P. isolated from drinking water hospitalized patients Jordan. A total 16 isolates obtained 15 bottled used study. Bacterial isolation identification was performed using routine microbiological methods confirmed PCR technique targeting 16S rDNA gene. determined by measuring minimum inhibitory concentration (MIC) 2-fold microdilution method. Synergy interaction between various antimicrobials checkerboard method fractional index (FICI). majority sensitive gentamicin (93.3%), ticarcillin (86.7%) ciprofloxacin, levofloxacin, amikacin, colistin, piperacillin, azlocillin, aztreonam, ceftazidime imipenem (100% each). All (100%) resistant amoxicillin, oxytetracycline doxycycline (93.3% 86.7, respectively). For clinical isolates, all ceftazidime, 81.3% while 62.5% gentamicin, ticracillin, imipenem. oxytetracycline, amoxicillin. Analysis synergy assay multi-drug (n=26) showed synergism (P ≤ 0.05) when ciprofloxacin or included combination. There no differences activity levofloxacin combined other agents beta-lactams aminoglycosides classes. activities beta lactams – aminoglycoside fluoroquinolone combinations. Results indicate alarming widespread presence multidrug-resistant associated chronic suppurative infections apparently clean Treatment lesions must be based on culture testing potent avoid emergence strains improve outcome treated patients.