Acute ethanol alters calcium signals elicited by glutamate receptor agonists and K+ depolarization in cultured cerebellar Purkinje neurons.

摘要: Abstract The effect of acute ethanol on Ca2+ signals evoked by ionotropic (iGluR) and metabotropic (mGluR) glutamate receptor (GluR) activation K+ depolarization was examined in cultured rat cerebellar Purkinje neurons to assess the sensitivity these signaling pathways. Mature ∼3 weeks vitro were studied. iGluRs activated (RS)-α-amino-3-hydroxyl-5 methyl-4-isoxazolepropionic acid (AMPA; 1 5 μM) domoate (5 μM). mGluRs (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic (ACPD; 300 (R,S)-3,5-dihydroxyphenylglycine (DHPG; 200 These agents (150 mM) applied from micropipettes brief (1 s) microperfusion pulses. levels monitored at 2–3 s intervals during pre- post-stimulus periods using microscopic digital imaging sensitive dye fura-2. iGluR mGluR agonists produced abrupt increases intracellular that slowly recovered baseline resting levels. Acute exposure 33 mM mg%) 66 (300 significantly reduced amplitude with little or no agonists. In contrast, 10 (45 had agonist AMPA but enhanced DHPG. results show modulates linked GluR a subtype specific manner, suggest pathways membrane may be important mechanisms which alters transduction excitatory synaptic glutamatergic synapses thereby affects intercellular communication CNS.