Simultaneous determination of volatile and non-volatile acidic fermentation products of anaerobes by capillary gas chromatography

摘要: A method previously used for the analysis of organic acids in silage has been applied to detection and quantification acidic fermentation products (C1 C6 volatile fatty acids, lactic succinic acid) rumen bacteria anaerobic fungi grown pure culture. The were converted tertiary butyldimethylsilyl derivatives prior separation on a 30 m DB1 capillary gas chromatographic column. quantitative recoveries formic found be comparable other reported original study. recovery acid was dependent storage samples at ambient temperature least 24 h following derivatization. simultaneous determination wide range non-volatile is an important feature this method.