Preclinical pharmacokinetics of M10 after intragastrical administration of M10-H and M10-Na in Wistar rats

摘要: Abstract As a myricetin derivative, M10 is potent agent of anti-chronic colonic inflammation. It has better activity than in preventing azoxymethane/dextran sulfate sodium - induced ulcerative colitis. Here, we introduce sensitive quantification method based on ultra performance liquid chromatography-tandem mass spectrometry for the determination M10-H and M10-Na Wistar rat plasma. Samples were treated with L ascorbic acid phosphate buffer solution to maintain stability acetonitrile remove proteins The supernatant was separated BEH C18 column eluted ultrapure water both containing 0.1% formic acid. detection performed by triple quadrupole spectrometer positive electrospray ionization mode multiple reactive monitoring. This validated carryover effect, selectivity, accuracy, precision, matrix stability, recovery. A linear correlation established between concentration response calibration curves over 10–2000 ng·mL−1 (r > 0.99). applied pharmacokinetic study intragastrical administration rats. In addition, relative bioavailability rats 60 ± 19%, calculated ratio area under (AUC) after single dose (100 mg·kg−1 M10-Na, respectively)