Three-dimensional reconstruction of whole cells of Streptococcus faecalis from thin sections of cells.

摘要: A new ultrastructural technique has been developed to study the geometry of cell wall assembly in Streptococcus faecalis, which is believed occur between pairs raised bands located on organism's surface. Three-dimensional reconstructions these regions envelope growth are produced from mathematical rotation (around a central axis) various measurements taken central, longitudinal thin sections cells. These can be used calculate surface area and volume septal peripheral walls that were supposedly present any given before sectioning. In an accompanying paper, it shown how such estimations, coupled with other length, thickness, curvature, characterize cycle this organism. The validity assumptions reconstruct cells by possible sources error using discussed.