Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing.
作者： Yifan Li , Zhenquan Lin , Can Huang , Yan Zhang , Zhiwen Wang
DOI: 10.1016/J.YMBEN.2015.06.006
关键词:
摘要: … Here, we report the development of a CRISPR–Cas9 based method for iterative genome editing and metabolic engineering of Escherichia coli. This system enables us to introduce …
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