MicroRNA-34a Modulates Chemosensitivity of Breast Cancer Cells to Adriamycin by Targeting Notch1
作者： Xiu-juan Li , Ming-hua Ji , Shan-liang Zhong , Quan-bing Zha , Jin-jin Xu
DOI: 10.1016/J.ARCMED.2012.09.007
关键词:
摘要: Background and Aims MicroRNA-34a (miR-34a) as a tumor suppressor has been reported in many other studies. However, its role modulating the sensitivity of breast cancer cells to adriamycin (ADR) remains unclear. The aim this study is evaluate miR-34a ADR. Methods was detected using MTT assay, flow cytometry real-time PCR Western blot, etc. association Notch1 analyzed by dual-luciferase reporter assay Notch1-siRNA technology. Real-time performed test expression 38 selective tissue samples. Results Ectopic overexpression could sensitize MCF-7 MiR-34a mimic inhibit luciferase activity construct containing wild-type 3′ UTR MCF-7/ADR cells. partially reverse effect inhibitor inducing chemoresistance Further, there an inverse between cancer. Conclusion Dysregulation plays critical roles acquired ADR resistance cancer, at least part via targeting Notch1.
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