Measurement of adenosine triphosphate and some other metabolites in blood cells by isotachophoresis. I. Preparative technique and enzymatic confirmation.

摘要: This study investigated the optimal conditions for detection of nucleotides in blood using an IP-1B capillary isotachophoretic apparatus. The system used 10 mM HCl-beta-alanine (pH 4.2) as leading electrolyte and n-caproic acid terminal electrolyte. Direct application lysed red cells was shown to be inaccurate, a method deproteinization based on heat microwave oven developed. zones 2,3-diphosphoglycerate, ATP, inorganic phosphate, lactate were identified enzymatically by withdrawal pure samples each zone via special cell. quantitative values obtained isotachophoresis also confirmed enzymatically. technique is now available convenient accurate identification these metabolites simultaneously.