Concentration of glycosaminoglycan in ovariectomized mice uterus after treatment with ovarian steroids.

摘要: The aim of this study was to evaluate the amount non- and sulfated glycosaminoglycans in ovariectomized mice uterus, after treatment with ovarian steroids. For purpose, 50 adult female were divided into five groups 10 animals/each: control group: CG (ovary intact), groups: OG (vehicle), EG (estradiol), PG (progesterone) EPG (estradiol combined progesterone). treatments started 30 days ovariectomy. All animals treated for consecutive days. These hormones administered a sterile oily solution via gavage. Twenty-four hours last treatment, all euthanized, removing uterine horn biochemical analyses. To quantify, hyaluronic acid (HA) used ELISA-like fluorometric assay, (GAGs) agarose gel electrophoresis. HA significantly higher group progesterone (PG) compared others (p < 0.05), estradiol (EG), chondroitin/dermatan sulfate (PG), heparan lower groups, except (p < 0.05). Our results showed that estroprogestative therapy long time (50 days) profoundly affected uterine. changes may be indicative pathology such as development tumor.