Rapid isolation of mouse ILCs from murine intestinal tissues.
作者： Kyle Burrows , Pailin Chiaranunt , Louis Ngai , Arthur Mortha
DOI: 10.1016/BS.MIE.2019.10.001
关键词:
摘要: Tissue-resident immune cells like innate lymphoid (ILCs) are regulators of local immunity and tissue homeostasis. Similar to Natural Killer (NK) cells, ILCs express germline-encoded natural cytotoxicity receptors (NCRs) that facilitate the rapid execution effector functions. Recent advances using transgenic animal models have further uncovered developmental, transcriptional, epigenetic, functional differences between members ILC family. Isolation ILCs, which particularly enriched in non-lymphoid tissues, can often be challenging time consuming. Here, we provide a simple protocol for isolation NK from murine intestinal tissues. This is suitable Fluorescence Activated Cell Sorting (FACS) intracellular analysis cytokine transcription factor expression flow mass cytometry.
sci-hub.se 参考文章(71)
Qi Yang, Fengyin Li, Christelle Harly, Shaojun Xing, Longyun Ye, Xuefeng Xia, Haikun Wang, Xinxin Wang, Shuyang Yu, Xinyuan Zhou, Maggie Cam, Hai-Hui Xue, Avinash Bhandoola, TCF-1 upregulation identifies early innate lymphoid progenitors in the bone marrow Nature Immunology. ,vol. 16, pp. 1044- 1050 ,(2015) , 10.1038/NI.3248
Yoshifumi Yokota, Ahmed Mansouri, Seiichi Mori, Seiichi Sugawara, Satoko Adachi, Shin-Ichi Nishikawa, Peter Gruss, Development of peripheral lymphoid organs and natural killer cells depends on the helix–loop–helix inhibitor Id2 Nature. ,vol. 397, pp. 702- 706 ,(1999) , 10.1038/17812
Stephanie L Sanos, Viet L Bui, Arthur Mortha, Karin Oberle, Charlotte Heners, Caroline Johner, Andreas Diefenbach, RORγt and commensal microflora are required for the differentiation of mucosal interleukin 22–producing NKp46 + cells Nature Immunology. ,vol. 10, pp. 83- 91 ,(2009) , 10.1038/NI.1684
Jochem H. Bernink, Lisette Krabbendam, Kristine Germar, Esther de Jong, Konrad Gronke, Michael Kofoed-Nielsen, J. Marius Munneke, Mette D. Hazenberg, Julien Villaudy, Christianne J. Buskens, Willem A. Bemelman, Andreas Diefenbach, Bianca Blom, Hergen Spits, Interleukin-12 and -23 Control Plasticity of CD127(+) Group 1 and Group 3 Innate Lymphoid Cells in the Intestinal Lamina Propria. Immunity. ,vol. 43, pp. 146- 160 ,(2015) , 10.1016/J.IMMUNI.2015.06.019
Sylvestre Chea, Cécilie Possot, Thibaut Perchet, Maxime Petit, Ana Cumano, Rachel Golub, CXCR6 Expression Is Important for Retention and Circulation of ILC Precursors Mediators of Inflammation. ,vol. 2015, pp. 368427- 368427 ,(2015) , 10.1155/2015/368427
Lu Wang, Yan Wang, Zhiyu Song, Jiahui Chu, Xianjun Qu, Deficiency of Interferon-Gamma or Its Receptor Promotes Colorectal Cancer Development Journal of Interferon & Cytokine Research. ,vol. 35, pp. 273- 280 ,(2015) , 10.1089/JIR.2014.0132
Michael C. Abt, Brittany B. Lewis, Silvia Caballero, Huizhong Xiong, Rebecca A. Carter, Bože Sušac, Lilan Ling, Ingrid Leiner, Eric G. Pamer, Innate Immune Defenses Mediated by Two ILC Subsets Are Critical for Protection against Acute Clostridium difficile Infection. Cell Host & Microbe. ,vol. 18, pp. 27- 37 ,(2015) , 10.1016/J.CHOM.2015.06.011
Susanne J Szabo, Sean T Kim, Gina L Costa, Xiankui Zhang, C.Garrison Fathman, Laurie H Glimcher, A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell. ,vol. 100, pp. 655- 669 ,(2000) , 10.1016/S0092-8674(00)80702-3
Victor S. Cortez, Michelle L. Robinette, Marco Colonna, Innate lymphoid cells: new insights into function and development Current Opinion in Immunology. ,vol. 32, pp. 71- 77 ,(2015) , 10.1016/J.COI.2015.01.004
Jarrod A. Dudakov, Alan M. Hanash, Robert R. Jenq, Lauren F. Young, Arnab Ghosh, Natalie V. Singer, Mallory L. West, Odette M. Smith, Amanda M. Holland, Jennifer J. Tsai, Richard L. Boyd, Marcel R. M. van den Brink, Interleukin-22 drives endogenous thymic regeneration in mice Science. ,vol. 336, pp. 91- 95 ,(2012) , 10.1126/SCIENCE.1218004