Ebola Virus Genome Plasticity as a Marker of Its Passaging History: A Comparison of In Vitro Passaging to Non-Human Primate Infection
作者： Jeffrey R. Kugelman , Michael S. Lee , Cynthia A. Rossi , Sarah E. McCarthy , Sheli R. Radoshitzky
DOI: 10.1371/JOURNAL.PONE.0050316
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摘要: To identify polymorphic sites that could be used as biomarkers of Ebola virus passage history, we repeatedly amplified (Kikwit variant) in vitro and vivo performed deep sequencing analysis the complete genomes viral subpopulations. We then determined undergoing selection during Vero E6 cells. Four locations within Kikwit genome were identified together segregate cell culture-passaged obtained from infected non-human primates. Three are located glycoprotein gene (GP) sequence: poly-U (RNA editing) site at position 6925, well positions 6677, 6179. One was found VP24 10833. In all cases, vivo, both populations (majority minority variants) maintained swarm, with rapid selections occurring after a few passages or infections. This approach will useful to differentiate whether filovirus stocks unknown history have been passaged culture may support stock standardization for medical countermeasure development.
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