Unraveling avian and reptilian hematology: An optical and electron microscopic study of the buffy coat.

摘要: BACKGROUND Blood centrifugation and buffy coats are at the cornerstone of hematology. In mammals, coat has a layered disposition (from bottom to top) with neutrophils on top erythrocytes, followed by monocytes/lymphocytes, platelets. nonmammals, this distribution is unknown. Recently, cell tube block (CTB) technique was developed study coat, but it never applied nonmammal coats. OBJECTIVES This aimed evaluate using CTB reptilian avian propose its use for clinical applications. METHODS from five birds eight reptiles different species obtained make CTBs that were processed optical/electron microscopy. H&E, Sirius red, immunohistochemistry staining against CD3 (to label T lymphocytes) CTBs. RESULTS birds, had appearance granulocyte layer containing granulocytes (heterophils eosinophils) nucleated erythrocytes mononuclear lymphocytes, monocytes, thrombocytes. some animals, erythrocyte observed admixed granulocyte/mononuclear layer. A small clot within seen in seven reptiles, less definition layers occurred only one or two layers. Lymphocytes appeared toward coat. CONCLUSIONS From comparative hematology perspective, mammals differs more reptiles. The can be used these differences hematology, especially atypical circulating cells, hemoparasites, blood proportions health disease.