Purification and characterization of the NADH-glutamate synthase from Chlamydomonas reinhardii
作者： Antonio J. Márquez , Francisco Galván , José M. Vega
DOI: 10.1016/S0304-4211(84)80010-3
关键词:
摘要: Summary Chlamydomonas cells contain two enzymes with glutamate synthase (GOGAT) activity, which are specific, respectively, for reduced pyridine nucleotide (NADH) or ferredoxin, as reductant. These enzymes, may also use methyl viologen electron donor, can be separated by ion exchange chromatography on DEAE-sephacel. The NADH-GOGAT has been purified 350-fold a method that uses affinity blue-sepharose. enzymatic complex keeps associated additional activities separately assayed; one is an NADH-diaphorase, ferricyanide acceptor, and the other (MVH)-GOGAT, cannot benzyl (BVH) flavins carrier. specific glutamine 2-oxoglutarate amido group donor respectively. Azaserine 6-diazo-5-oxo-L-norleucine (DON), at 5 mM, inhibit NADH- MVH-GOGAT but not NADH-diaphorase activity of complex. p -Hydroxymercuribenzoate ( -HMB) strongly inhibits all complex, more resistant than NADH-GOGAT.
elsevier.com
researchgate.net 
sci-hub.se 参考文章(24)
John Leyden. Webb, Enzyme and metabolic inhibitors Academic Press. ,(1963) , 10.5962/BHL.TITLE.7320
Yasuhiro Arima, Glutamate synthase in rice root extracts and the relationship among electron donors, nitrogen donors and its activity Plant and Cell Physiology. ,vol. 19, pp. 955- 961 ,(1978) , 10.1093/OXFORDJOURNALS.PCP.A075685
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Toru Matoh, Fukuko Suzuki, Shoji Ida, Corn leaf glutamate synthase: Purification and properties of the enzyme Plant and Cell Physiology. ,vol. 20, pp. 1329- 1340 ,(1979) , 10.1093/OXFORDJOURNALS.PCP.A075932
Toru Matoh, Eiichi Takahashi, Shoji Ida, Glutamate synthase in developing pea cotyledons: Occurrence of NADH-dependent and ferredoxin-dependent enzymes Plant and Cell Physiology. ,vol. 20, pp. 1455- 1459 ,(1979) , 10.1093/OXFORDJOURNALS.PCP.A075947
Glutamate Synthase in Greening Pea Shoots Plant and Cell Physiology. ,vol. 22, pp. 727- 731 ,(1981) , 10.1093/OXFORDJOURNALS.PCP.A076217
Leonard Beevers, Richard Storey, Glutamate Synthetase in Developing Cotyledons of Pisum sativum Plant Physiology. ,vol. 57, pp. 862- 866 ,(1976) , 10.1104/PP.57.6.862
P.J. Lea, B.J. Miflin, The occurrence of glutamate synthase in algae. Biochemical and Biophysical Research Communications. ,vol. 64, pp. 856- 862 ,(1975) , 10.1016/0006-291X(75)90126-6
T. Matoh, S. Ida, E. Takahashi, Isolation and characterization of NADH-glutamate synthase from pea (Pisum sativum L.). Plant and Cell Physiology. ,vol. 21, pp. 1461- 1474 ,(1980) , 10.1093/PCP/21.8.1461
Roger M. Wallsgrove, Peter J. Lea, Benjamin J. Miflin, The development of NAD(P)H-dependent and ferredoxin-dependent glutamate synthase in greening barley and pea leaves. Planta. ,vol. 154, pp. 473- 476 ,(1982) , 10.1007/BF01267816