Hyaluronic acid and alginate covalent nanogels by template cross-linking in polyion complex micelle nanoreactors.

摘要: Abstract Hyaluronic acid (HA) and alginate (AL) covalent nanogels cross-linked with l -lysine ethyl ester were prepared by template chemical cross-linking of the polysaccharide in polyion complex micelle (PIC) nanoreactors. By using this method we able to prepare HA AL without organic solvents. PICs poly(ethylene oxide)-block-poly[(3-acrylamidopropyl)-trimethylammonium chloride] (PEO-b-PAMPTMA) or poly[(N-isopropylacrylamide)-block-PAMPTMA] (PNIPAAM-b-PAMPTMA). Only PNIPAAM-b-PAMPTMA block copolymers allowed PIC small controlled size. Short chains (Xn = 50 63 for HA, respectively, where Xn is number monosaccharidic units present polysaccharide) used optimize formation. The remarkable difference charge density rigidity did not have a significant influence on formation PICs. size (diameter about 50–80 nm) low polydispersity obtained up 5 mg/mL polymer. After ester, nanoreactors dissociated adding NaCl. easily purified isolated dialysis. dissociation confirmed 1H NMR, DLS, TEM ζ-potential measurements. smallest solution swollen state was 50–70 nm presence salt 80–100 nm water.