A novel functionalisation process for glucose oxidase immobilisation in poly(methyl methacrylate) microchannels in a flow system for amperometric determinations.

摘要: Different materials like glass, silicon and poly(methyl methacrylate) (PMMA) are being used to immobilise enzymes in microchannels. PMMA shows advantages such as its low price, biocompatibility attractive mechanical chemical properties. Despite this, the introduction of reactive functional groups on is still problematic, either because complex chemistry or extended reaction time involved. In this paper, a new methodology was developed glucose oxidase (GOx) microchannels, with benefit rapid immobilisation process very simple route. The procedure involves only two steps, based 5.0% (w/w) polyethyleneimine (PEI) dimethyl sulphoxide medium, followed by using solution containing 100U 1.0% (v/v) glutaraldehyde. reactors prepared way were evaluated flowing system amperometric detection (+0.60V) oxidation H2O2 produced reactor. microreactor proposed here able work high bioconversion frequency 60 samples h(-1), quantification limits 0.50 1.66µmol L(-1), respectively. Michaelis-Menten parameters (Vmax KM) calculated 449±47.7nmol min(-1) 7.79±0.98mmol. Statistical evaluations done validate methodology. content natural commercial coconut water method. Comparison spectrophotometric measurements showed that both methodologies have good correlation (tcalculated, 0.05, 4=1.35