Deep Sequencing Analysis of the Ixodes ricinus Haemocytome

摘要: Background Ixodes ricinus is the main tick vector of microbes that cause Lyme disease and tick-borne encephalitis in Europe. Pathogens transmitted by ticks have to overcome innate immunity barriers present tissues, including midgut, salivary glands epithelia hemocoel. Molecularly, invertebrate initiated when pathogen recognition molecules trigger serum or cellular signalling cascades leading production antimicrobials, opsonization phagocytosis. We presently aimed at identifying hemocyte transcripts from semi-engorged female I. mass sequencing a cDNA library annotating immune-related based on their abundance as well ubiquitous distribution. Methodology/principal findings De novo assembly 926,596 pyrosequence reads plus 49,328,982 Illumina (148 nt length) library, together with over 189 million gland midgut libraries, generated 15,716 extracted coding sequences (CDS); these are displayed an annotated hyperlinked spreadsheet format. Read mapping allowed identification annotation tissue-enriched transcripts. A total 327 were found significantly expressed those for scavenger receptors, antimicrobial peptides, proteins, proteases protease inhibitors. Vitellogenin lipid metabolism transcription enrichment suggests fat body components. additionally ubiquitously distributed associated immune function, immune-associated signal transduction proteins factors, STAT factor. Conclusions/significance This first systems biology approach describe genes haemocytes this neglected vector. 2,860 deposited GenBank, increasing 27,547 number so far our previous transcriptome studies serves discovery platform biochemistry physiology.