Identification of ubiquitin conjugated Protein Phosphatase Inhibitor 1 from postmortem bovine muscle
作者： Purevdorj Nyam-Osor , Barana Jayawardana , Juan Marcos Aro Aro , Ken-ichiro Shimada , Michihiro Fukushima
DOI: 10.1016/J.FOODCHEM.2009.01.061
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摘要: Abstract Previously, we characterised the presence of ubiquitin and some ubiquitinated proteins (conjugates), which were considered as conjugates in sarcoplasmic fraction postmortem skeletal cardiac muscles using SDS–PAGE Western blot. In this study, an conjugate around 27 kDa was subjected to identification. The N-terminal amino acid sequence analysis generated by Edman degradation resulted a 13 (KIQFTVPLLEPHL), is similar that Protein Phosphatase Inhibitor 1. Based on result, blot performed polyclonal antibody for 1 recognised reacted clearly. Thus, protein identified
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