Flow-assisted allergy diagnosis: current applications and future perspectives.

摘要: Physicians predominantly rely upon quantification of serum-specific immunoglobulin E (IgE) and/or skin test to confirm clinically suspected IgE-mediated allergy. However, for various reasons, identification the offending allergen(s) and potentially cross-reactive structures is not always straightforward. Flow-assisted allergy diagnosis relies alterations in expression particular basophilic activation markers. Actually, challenge with a specific allergen, basophils only secrete quantifiable bioactive mediators but also upregulate different markers which can be detected efficiently by flow cytometry using monoclonal antibodies. Currently, technique has been applied investigation caused classical inhalant allergens, food, Hevea latex, hymenoptera venoms drugs. It appreciated; proves valuable non-IgE-mediated (anaphylactoid) reactions such drug hypersensitivity detection autoantibodies certain forms chronic urticaria. This review will address immunologic features, characteristics general pitfalls flow-assisted analysis vitro-activated as summarized elsewhere. After recapitulation principles some technical issues basophils, we principally focus on current clinical research applications basophil tests. Personal experience both groups provided, where appropriate. Finally, viewpoint how field might evolve following years provided.