Spt6 is required for heterochromatic silencing in the fission yeast Schizosaccharomyces pombe
作者： C. M. Kiely , S. Marguerat , J. F. Garcia , H. D. Madhani , J. Bahler
DOI: 10.1128/MCB.05568-11
关键词:
摘要: Spt6 is a conserved factor, critically required for several transcription- and chromatin-related processes. We now show that its binding partner, Iws1, are heterochromatic silencing in Schizosaccharomyces pombe. Our studies demonstrate of all loci an spt6 mutant has unusual combination phenotypes compared to previously studied mutants. Unexpectedly, we find normal nucleosome positioning over heterochromatin levels histone H3K9 dimethylation at the endogenous pericentric repeats. However, also greatly reduced trimethylation, elevated H3K14 acetylation, recruitment factors, defects spreading. evidence suggests plays role both transcriptional posttranscriptional levels; mutant, RNA polymerase II (RNAPII) occupancy regions only modestly increased, while production small interfering RNAs (siRNAs) lost. Taken together, our results suggest multiple steps by controlling chromatin, transcriptional, from prokaryotes (49) humans (25) many roles regulation transcription chromatin structure. Studies organisms, particularly Saccharomyces cerevisiae, have elucidated functions.
ucsf.edu 参考文章(100)
Shane M Buker, Tetsushi Iida, Marc Bühler, Judit Villén, Steven P Gygi, Jun-Ichi Nakayama, Danesh Moazed, Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast Nature Structural & Molecular Biology. ,vol. 14, pp. 200- 207 ,(2007) , 10.1038/NSMB1211
D Kipling, S E Kearsey, Reversion of autonomously replicating sequence mutations in Saccharomyces cerevisiae: creation of a eucaryotic replication origin within procaryotic vector DNA. Molecular and Cellular Biology. ,vol. 10, pp. 265- 272 ,(1990) , 10.1128/MCB.10.1.265
Ken-ichi Noma, Tomoyasu Sugiyama, Hugh Cam, Andre Verdel, Martin Zofall, Songtao Jia, Danesh Moazed, Shiv I S Grewal, RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing. Nature Genetics. ,vol. 36, pp. 1174- 1180 ,(2004) , 10.1038/NG1452
Craig D Kaplan, James R Morris, C-T Wu, Fred Winston, Spt5 and Spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster Genes & Development. ,vol. 14, pp. 2623- 2634 ,(2000) , 10.1101/GAD.831900
V Wood, R Gwilliam, M-A Rajandream, M Lyne, R Lyne, A Stewart, J Sgouros, N Peat, J Hayles, S Baker, D Basham, S Bowman, K Brooks, D Brown, S Brown, T Chillingworth, C Churcher, M Collins, R Connor, A Cronin, P Davis, T Feltwell, A Fraser, S Gentles, A Goble, N Hamlin, D Harris, J Hidalgo, G Hodgson, S Holroyd, T Hornsby, S Howarth, EJ Huckle, S Hunt, K Jagels, K James, L Jones, M Jones, S Leather, S McDonald, J McLean, P Mooney, S Moule, K Mungall, L Murphy, D Niblett, C Odell, K Oliver, S O'neil, D Pearson, MA Quail, E Rabbinowitsch, K Rutherford, S Rutter, D Saunders, K Seeger, S Sharp, J Skelton, M Simmonds, R Squares, S Squares, K Stevens, K Taylor, RG Taylor, A Tivey, S Walsh, T Warren, S Whitehead, J Woodward, G Volckaert, R Aert, J Robben, B Grymonprez, I Weltjens, E Vanstreels, M Rieger, M Schäfer, S Müller-Auer, C Gabel, M Fuchs, C Fritzc, E Holzer, D Moestl, H Hilbert, K Borzym, I Langer, A Beck, H Lehrach, R Reinhardt, TM Pohl, P Eger, W Zimmermann, H Wedler, R Wambutt, B Purnelle, A Goffeau, E Cadieu, S Dreano, S Gloux, V Lelaure, S Mottier, F Galibert, SJ Aves, Z Xiang, C Hunt, K Moore, SM Hurst, M Lucas, M Rochet, C Gaillardin, VA Tallada, A Garzon, G Thode, RR Daga, L Cruzado, J Jimenez, M Sanchez, F Del Rey, J Benito, A Dominguez, JL Revuelta, S Moreno, J Armstrong, SL Forsburg, L Cerrutti, T Lowe, WR McCombie, I Paulsen, J Potashkin, GV Shpakovski, David Ussery, BG Barrell, P Nurse, None, The genome sequence of Schizosaccharomyces pombe Nature. ,vol. 415, pp. 871- 880 ,(2002) , 10.1038/NATURE724
D. L. Lindstrom, S. L. Squazzo, N. Muster, T. A. Burckin, K. C. Wachter, C. A. Emigh, J. A. McCleery, J. R. Yates, G. A. Hartzog, Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Molecular and Cellular Biology. ,vol. 23, pp. 1368- 1378 ,(2003) , 10.1128/MCB.23.4.1368-1378.2003
Marianna Wirén, Rebecca A Silverstein, Indranil Sinha, Julian Walfridsson, Hang-mao Lee, Patricia Laurenson, Lorraine Pillus, Daniel Robyr, Michael Grunstein, Karl Ekwall, Genome wide analysis of nucleosome density histone acetylation and HDAC function in fission yeast The EMBO Journal. ,vol. 24, pp. 2906- 2918 ,(2005) , 10.1038/SJ.EMBOJ.7600758
E A Malone, C D Clark, A Chiang, F Winston, Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae. Molecular and Cellular Biology. ,vol. 11, pp. 5710- 5717 ,(1991) , 10.1128/MCB.11.11.5710
M S Swanson, M Carlson, F Winston, SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus Molecular and Cellular Biology. ,vol. 10, pp. 4935- 4941 ,(1990) , 10.1128/MCB.10.9.4935
Devin Close, Sean J. Johnson, Matthew A. Sdano, Seth M. McDonald, Howard Robinson, Tim Formosa, Christopher P. Hill, Crystal structures of the S. cerevisiae Spt6 core and C-terminal tandem SH2 domain. Journal of Molecular Biology. ,vol. 408, pp. 697- 713 ,(2011) , 10.1016/J.JMB.2011.03.002