Expression and activity of Runx2 mediated by hyaluronan during chondrocyte differentiation
作者： Y. Tanne , K. Tanimoto , N. Tanaka , M. Ueki , Y.Y. Lin
DOI: 10.1016/J.ARCHORALBIO.2007.12.007
关键词:
摘要: During endochondral ossification, the production of hyaluronan (HA) is strictly and selectively regulated by chondrocytes, with a temporal peak at hypertrophic stage. This study was conducted to clarify effects HA on expression activity runt-related gene 2 (Runx2), potent transcription factor for chondrocyte differentiation in chondrocytes. Immature chondrocytes from an ATDC5 cell line were cultured differentiated DMEM/Ham's F12 pre-defined supplements. Using real-time PCR, expressions type II collagen, MMP-13, HAS2, Runx2 analysed days 0 18 differentiation. The after treatment oligosaccharide (HAoligo) using AML-3/Runx2 binding, PCR Western blot analysis. pre-incubation anti-CD44 antibody also examined. Expression X collagen mRNAs reached maximum terminal significantly inhibited treated HAoligo compared untreated controls. High molecular weight-HA did not affect or Runx2. mRNA decreased antibody. These results suggest that may during ossification inhibiting
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