Determination of Serum and Urine Proteins by Electrophoresis and Its Importance in Clinical Veterinary Medicine

摘要: In this study, for the investigation of routine clinical usage urine protein electrophoresis in laboratory to compare with findings veterinary medicine, qualitative and quantitative changes patterns serum from dogs kidney disorders proteinuria were investigated using electrophoresis. Eighteen (patient group)of various ages breeds proved clinic seventeen healthy (control group)were used study. The histories (age,sex,breed)of control patient groups recorded clinic.The blood was collected cephalic vein,and removed a centrifuge.Urine obtained by sterile catheter ureter.Biochemical analysis performed urines centrifuged ultrafiltrated concentrate Serum proteins separated 10 %SDS-PAGE. Creatinine detected Jaffe reaction, urea total modified Gentzkow biuret methods respectively. A/G ratios protein/creatinine calculated arithmetically. Levels creatinine found be high statistically significant (p