A highly sensitive automated complex running wheel test to detect latent motor deficits in the mouse MPTP model of Parkinson's disease.

摘要: The subchronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) paradigm is one of the most widely used in vivo models Parkinson's disease (PD). However, particularly mouse model it has remained difficult to reliably detect behavioural correlates for PD. In present study we apply a novel murine motor test, skill sequence (MOSS) based on computerized recording voluntary running wheel activity, and found latent deficits MPTP model. Mice are first subjected 2-week training phase. animals then receive either injections according standard (30 mg/kg) or vehicle 5 consecutive days. Running performance transiently fell during injection phase but returned baseline within few were exposed complex wheels with irregularly spaced crossbars demanding high central coordination abilities. Though both groups showed clear improvement learning wheel, displayed wheels, as indicated by reduced maximum speed distance, despite unchanged motivation. Our results demonstrate MPTP-treated mice, which can be unmasked MOSS. MOSS thus capable detecting quantifying this PD sensitivity. automated full time data collection several different parameters makes also suitable test efficient screening potential therapeutic compounds