A sensitive method for immunocytochemical detection of P-glycoprotein in multidrug-resistant human ovarian carcinoma cell lines.

摘要: P-glycoprotein, a molecular weight 170 kilodalton membrane component can be accurately detected in series of human ovarian carcinoma cells with increasing degrees multidrug resistance by using modified immunoperoxidase "sandwich" method. Drug-resistant derivatives were selected from drug-sensitive parent cell line, SKOV3, continuous exposure to concentrations the cytotoxic drug vincristine. These had corresponding overexpression P-glycoprotein demonstrable at both protein and mRNA levels. Monoclonal antibodies against localized staining for plasma Golgi region individual drug-resistant cells, proportion their expression. was not SKOV3 either immunoblotting or immunocytochemical methods. The method allowed detection least line as low 8-fold relative This is sensitive Northern blot, more than standard Western blot P-glycoprotein. We conclude that this highly suitable determination expression biopsy samples tumors, it powerful diagnostic prognostic tool study natural history resistance. may have important applications clinical management cancer chemotherapy.